Journal: International Journal of Clinical and Experimental Pathology

Article Title: Urinary microRNAs as potential biomarkers for differentiating the “atypical urothelial cells” category of the Paris system for reporting urine cytology

doi:

Figure Lengend Snippet: The expression of microRNAs in the cell lines. The expression of miR-182, miR-183, and miR-96 increased, whereas the expression of miR-145, miR-1, miR-133a, miR-99a, miR-125b, miR-195, miR-100, and miR-29c decreased in bladder tumor cell lines compared to normal human urothelial tissue. (RQ, relative quantitation).

Article Snippet: Five human bladder urothelial tumor cell lines (RT4, T24, 5637, HT1376, and 253J) were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Quantitation Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Urinary microRNAs as potential biomarkers for differentiating the “atypical urothelial cells” category of the Paris system for reporting urine cytology

doi:

Figure Lengend Snippet: MicroRNAs associated with proliferative activity. A. The 5637 and T24 urothelial cancer cell lines revealed increased proliferative activity than 253J, HT1376, and RT4 cell lines in proliferation assay. B. The expression of miR-133a and miR-145 decreased as the proliferation increased. (RQ, relative quantitation).

Article Snippet: Five human bladder urothelial tumor cell lines (RT4, T24, 5637, HT1376, and 253J) were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Activity Assay, Proliferation Assay, Expressing, Quantitation Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Urinary microRNAs as potential biomarkers for differentiating the “atypical urothelial cells” category of the Paris system for reporting urine cytology

doi:

Figure Lengend Snippet: MicroRNAs expression in formalin-fixed and paraffin-embedded tissue of urothelial neoplasms. The expression of miR-145, miR-133a and miR-205 in formalin-fixed paraffin-embedded tissue. When whole section including stromal tissue was used (stroma-rich), the expression of miR-145 increased in invasive urothelial carcinoma. When microdissected specimens were used, the expression of miR-145 and miR-133a as what was expected. The expression of miR-205 showed similar trends as the cell lines. (RQ, relative quantitation; PUNLMP, papillary urothelial neoplasm of low malignant potential; NIUCLG, non-invasive low-grade urothelial carcinoma; NIUCHG, non-invasive high-grade urothelial carcinoma; INVASIVE, invasive urothelial carcinoma).

Article Snippet: Five human bladder urothelial tumor cell lines (RT4, T24, 5637, HT1376, and 253J) were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing, Formalin-fixed Paraffin-Embedded, Quantitation Assay

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Urinary microRNAs as potential biomarkers for differentiating the “atypical urothelial cells” category of the Paris system for reporting urine cytology

doi:

Figure Lengend Snippet: MicroRNA expression in the “atypical urothelial cells” category of the Paris System for Reporting Urine Cytology. When “atypical urothelial cells” was proven to be benign when followed up (abbreviated as AN), the expression of miR-99a increased as in the “negative” category urine (abbreviated N). When “atypical urothelial cells” was proven malignant when followed up (abbreviated as AU), the expression of miR-99a decreased as in the “malignant” category urine (abbreviated as UC).

Article Snippet: Five human bladder urothelial tumor cell lines (RT4, T24, 5637, HT1376, and 253J) were purchased from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Expressing

Journal: BMC Cancer

Article Title: Nectin-4 is a new histological and serological tumor associated marker for breast cancer

doi: 10.1186/1471-2407-7-73

Figure Lengend Snippet: Analysis of Nectin-4 expression in normal and tumor cells . A: Cell surface expression of Nectin-4 was assessed by FACS analysis using the N4.61 mAb. The level of cell surface expressed Nectin-4 (L) was calculated in arbitrary units based on fluorescence intensity: L = MFI N4.61/MFI control IgG1. Similar results were obtained with the N4.40 mAb. L = 1 corresponds to negative cell lines, 2 < L < 15: low expression, 16 < L < 50: intermediate expression, L > 50 high expression. These results are representative of at least three independant experiments. *, non cancerous breast cell lines. B: Nectin-4 expression level on breast tumor cell lines was monitored by FACS analysis using the anti-Nectin-4 N4.61 mAb (black line) and compared with a mouse irrelevant IgG1 (gray line). Examples of a negative cell line (MDA-MB-231) and two intermediate expressing cell lines (T47D and MCF-7). C: Correlation between Nectin-4 cell surface expression and Nectin-4 transcriptional expression. Quantitative PCR was performed on 17 breast tumor cell lines. Values were calculated as described in material and methods. The results are representative of at least two experiments.

Article Snippet: The bladder tumor cell lines were kindly provided by Dr. F. Radvanyi (Institut Curie, Paris, France).

Techniques: Expressing, Fluorescence, Control, Real-time Polymerase Chain Reaction